Detection of hepatitis B virus DNA in serum by a rapid filtration-hybridization assay
β Scribed by G. Morace; K. Von Der Helm; W. Jilg; F. Deinhardt
- Book ID
- 119114322
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 528 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0166-0934
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Factors determining the sensitivity and specificity of the spot-blot hybridization technique for the detection of hepatitis B virus DNA in serum were systematically investigated. Methods for pretreatment of serum samples, mode of application of the samples to the transfer membranes, blot treatment a
## Abstract An assay based on nucleic acid hybridization detects and quantitates hepatitis B virus (HBV) DNA in particles present in serum. This assay allows rapid examination of multiple samples and is sensitive and reproducible; serum samples are treated with proteolytic enzyme and detergent and