Detection of hepatitis B virus DNA and determination of surface antigen expression in peripheral blood mononuclear cells from patients with AIDS
✍ Scribed by C. Malavé Lara; M. T. Gorriño; C. Campelo; P. Lardelli; R. Cisterna
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 781 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0934-9723
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## Abstract The presence of both hepatitis B virus (HBV) DNA and HBV antigens (HBsAg, HBeAg) was assessed in peripheral blood mononuclear cells (PBMC) from 32 patients chronically infected with HBV. Three different molecular forms of HBV DNA were observed: free monomers (5), high‐molecular‐weight f
## Abstract The integration of hepatitis B virus (HBV) DNA in the liver of chronic HBV carriers has been documented extensively. However, the status of the viral genome during acute infection has not been assessed conclusively. While HBV DNA sequences are detected often in serum, liver, and periphe
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## Abstract Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglut