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Detection of hepatitis B viral DNA by polymerase chain reaction in patients with hepatitis B surface antigen

✍ Scribed by Ling-Na Shih; Jin-Chuan Sheu; Jin-Town Wang; Guan-Tarn Huang; Juei-Suei Chen; Juei-Low Sung; Teh-Hong Wang; Prof. Ding-Shinn Chen

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
690 KB
Volume
30
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Hepatitis B virus (HBV) DNA was assayed using the polymerase chain reaction in serum samples of 116 hepatitis B surface antigen (HBsAg) carriers, including 30 positive for hepatitis B e antigen (HBeAg) and 86 negative for HBeAg. In the HBeAg‐positive group, all were positive for HBV DNA. In the HBeAg‐negative group, 80.2% were positive for HBV DNA (80.0% in the healthy carrier group, 90.0% in the chronic active liver disease group, and 69.2% in patients with cirrhosis). This study indicated that every HBeAg‐positive carrier as well as the majority of HBeAg‐negative carriers were infectious and, in the latter group, that viral replication is most active in patients with chronic active liver disease.

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