Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t
β¦ LIBER β¦
Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction
β Scribed by S. Perelle; F. Dilasser; J. Grout; P. Fach
- Book ID
- 108837891
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 140 KB
- Volume
- 98
- Category
- Article
- ISSN
- 1364-5072
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## Abstract Sapovirus (SaV) is an agent of gastroenteritis for humans and swine, and is divided into five distinct genogroups (GIβGV) based on its capsid gene sequences. Typical methods of SaV detection include electron microscopy (EM), enzymeβlinked immunosorbent assay (ELISA), and reverse transcr