Detection of Escherichia coli O157:H7 and Listeria monocytogenes in Beef Products by Real-Time Polymerase Chain Reaction
โ Scribed by Nguyen, L.T.; Gillespie, B.E.; Nam, H.M.; Murinda, S.E.; Oliver, S.P.
- Book ID
- 121354240
- Publisher
- Mary Ann Liebert
- Year
- 2004
- Tongue
- English
- Weight
- 434 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1535-3141
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๐ SIMILAR VOLUMES
Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t
The effectiveness of low gamma-irradiation doses in the destruction of Escherichia coli O 157 : H7 and Listeria monocytogenes in raw beef sausages was investigated. Raw samples of fresh manufactured beef sausage were subjected to gamma-irradiation at doses of 0, 1, 2, and 3 kGy. Then samples were co