A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti
✦ LIBER ✦
Detection of enzymatic activities in sodium dodecyl sulfate-polyacrylamide gels: DNA polymerases as model enzymes
✍ Scribed by A. Blank; John R. Silber; Michael P. Thelen; Charles A. Dekker
- Book ID
- 107711946
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 875 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0003-2697
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