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Detection of Enterotoxigenic Escherichia coil in Foods by DNA Colony Hybridization

โœ Scribed by JOSEPH L. FERREIRA; WALTER E. HILL; MOSTAFA K. HAMDY; FRANCIS A. ZAPATKA; STEVEN G. McCAY


Book ID
108811691
Publisher
Institute of Food Technologists
Year
1986
Tongue
English
Weight
346 KB
Volume
51
Category
Article
ISSN
0022-1147

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A procedure is described for the detection of specific DNA sequences in Saccharomyces cerevisiae. This method allows a rapid screening of a large number of yeast colonies. The yeast cells of each colony, grown on nitrocellulose filters, are converted, in situ, to protoplasts by snail enzyme, and are