✦ LIBER ✦

Detection of specific DNA sequences in yeast by colony hybridization

✍ Scribed by Blanc, H. ;Dujon, B. ;Guerineau, M. ;Slonimski, P. P.

Publisher: Springer
Year: 1978
Tongue: English
Weight: 447 KB
Volume: 161
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331006

No coin nor oath required. For personal study only.

✦ Synopsis

A procedure is described for the detection of specific DNA sequences in Saccharomyces cerevisiae. This method allows a rapid screening of a large number of yeast colonies. The yeast cells of each colony, grown on nitrocellulose filters, are converted, in situ, to protoplasts by snail enzyme, and are then lysed and their DNAs are denatured and fixed on the filter. The presence of the specific DNA sequence is detected directly on the filter by hybridization with a radioactive cRNA. We have used successfully this technique to detect the presence or the absence of specific mt DNA sequences in p+, p- and p0 strains, and to detect the presence or the absence of the 2 mum DNA sequences in different strains.

📜 SIMILAR VOLUMES

Detection of virus-specific DNA sequence

Detection of virus-specific DNA sequences by quantitative in situ hybridization during infection with SV40

✍ Yves Langelier; Rosemonde Mandeville; René Simard 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 880 KB

## Abstract In situ hybridization has been used as a quantitative method to study the replication of SV40 during lytic infection. Various parameters have been defined such as fixation and denaturation of cytological preparations, concentration of complementary (^3^H)‐cRNA and volume of buffer used

Detection of legumin gene DNA sequences

Detection of legumin gene DNA sequences in pea by in situ hybridization

✍ Paul R. Simpson; Mari-Anne Newman; D. Roy Davies 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 983 KB

Enzyme-Based Multi-Component Optical Nan

Enzyme-Based Multi-Component Optical Nanoprobes for Sequence- Specific Detection of DNA Hybridization

✍ J. Li; S. Song; X. Liu; L. Wang; D. Pan; Q. Huang; Y. Zhao; C. Fan 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB 👁 2 views

Detection of EBV and HPV DNA sequences i

Detection of EBV and HPV DNA sequences in oral “hairy” leukoplakia by in situ hybridization

✍ Dr. L. R. Eversole; C. E. Stone; A. M. Beckman 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 559 KB

Oral "hairy" leukoplakia (OHL) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (HIV) positive acquired immunodeficiency syndrome (AIDS)-risk patients. The lesion has been reported to be associated with Epstein-Barr virus (EBV) and human

Detection of amplified dna sequences in

Detection of amplified dna sequences in human tumor cell lines by fluorescence in situ hybridization

✍ Irit Bar-Am; Orna Mor; Yosef Shiloh; Lydia Avivi; Herman Yeger 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 761 KB

An unambiguous and rapid characterization of amplified DNA sequences in tumor cells is important for the understanding of neoplastic progression. This study was conducted t o evaluate the potential of fluorescence in situ hybridization (FISH) t o identify such amplified DNA sequences in human tumor

A reproducible microanalytical method fo

A reproducible microanalytical method for the detection of specific RNA sequences by dot-blot hybridization

✍ Steve Cheley; Robert Anderson 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 884 KB

A rapid, microanalytical procedure for the reproducible isolation of RNA from small cultured cell samples and application to dot-blot hybridization is described. The procedure employs guanidine hydrochloride solubilization of whole cells, disruption by syringing, and selective precipitation of RNA w