Detection of DNA damage in mutagen-treated mammalian tissues by alkaline elution assay
โ Scribed by N. Hachiya; M. Sato; Y. Takizawa
- Book ID
- 113250714
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 81 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0165-1161
No coin nor oath required. For personal study only.
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A fluorometric procedure is described that can be used in the alkaline elution technique for the measurement of DNA damage in cells whose DNA is not, or cannot be, radioactively labeled. The procedure can be used for the measurement of DNA single-strand breaks, DNA-protein crosslinking, and DNA inte
Alkaline elution is a sensitive and commonly used technique to detect cellular DNA damage in the form of DNA strand breaks and DNA cross-links. Conventional alkaline elution procedures have extensive equipment requirements and are tedious to perform. Our laboratory recently presented a rapid, simpli