The development of a quadriplex PCR method with amplification of HCMV in a single-step procedure using primers taken from four different regions of the viral genome is described. Different concentrations of dNTPs and MgCl 2 were assayed in order to optimize the constitution of the buffer for the mul
Detection of cytomegalovirus in the meconium of infected newborns by polymerase chain reaction
β Scribed by Maria Esterlita T. Villanueva; David M. Svinarich; Bernard Gonik; Enrique M. Ostrea; Jr.
- Publisher
- Hindawi Publishing Corporation
- Year
- 2000
- Tongue
- English
- Weight
- 245 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1064-7449
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Two major variables may cause false-negative results in prenatal diagnosis of congenital human cytomegalovirus (HCMV) infection: sensitivity of the technique(s) used; and time elapsed between maternal infection and antenatal testing. Previous results indicated that rapid HCMV isolation from amniotic
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