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Detection of copper on polyacrylamide gels

✍ Scribed by W.J. Bruyninckx; S. Gutteridge; H.S. Mason

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 631 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90738-8

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✦ Synopsis

A color test for the localization of copper on polyacrylamide gels is described. The test is based upon the quenching of fluorescence of bathocuproine sulfonate by CtP and is sensitive to 0.1 nmol of free or protein-bound copper. There is no false positive reaction with 10 nmol of hemeprotein, free Fe3+, Fez+, Co*+, or MtP.

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