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Quantitative detection of orthophosphate in polyacrylamide gels

✍ Scribed by Alexander Baykov; Sergej Volk

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 196 KB
Volume: 151
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90045-4

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✦ Synopsis

A one-step procedure for the detection of &producing enzymes in polyacrylamide and agarose gels was developed using PPi hydrolysis by inorganic pyrophosphatase as a model reaction. The color reagent consists of acid molybdate, methyl green, and Triton X-305 and produces sharp greenish-blue bands in places of Pi accumulation. The color is stable and its intensity is linearily related to enzyme amounts in the gel. 0 1985 Academic press.

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