𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of circulating cancer cells expressing uroplakins and epidermal growth factor receptor in bladder cancer patients

✍ Scribed by Iman Osman; Melissa Kang; Andy Lee; Fang-Ming Deng; David Polsky; Maryann Mikhail; Caroline Chang; Dexter A. David; Nandita Mitra; Xue-Ru Wu; Tung-Tien Sun; Dean F. Bajorin

Publisher
John Wiley and Sons
Year
2004
Tongue
French
Weight
141 KB
Volume
111
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Our purpose was to determine the clinical relevance of the detection of circulating tumor cells (CTCs) expressing urothelial and epithelial markers in bladder cancer patients. Sixty‐two patients who presented to Memorial Sloan‐Kettering Cancer Center between July 2000 and September 2001 were studied. Peripheral blood was tested by nested RT‐PCR assay for uroplakins (UPs) Ia, Ib, II and III as well as for epidermal growth factor receptor (EGFR). We determined the sensitivity and specificity of each individual marker and the combinations of UPIa/UPII and UPIb/UPIII. The latter strategy was based on our data, which showed that UPIa and UPIb form heterodimers with UPII and UPIII, respectively. Forty patients had clinically advanced bladder cancer and 22 had no evidence of disease at the time of assay. Eight of the 22 patients recurred during the follow‐up period. All 8 patients were positive at presentation for UPIa/UPII. The combination of UPIa/UPII provided the best sensitivity (75%) of detecting CTCs, with a specificity of 50%. The combination of UPIb/UPIII was the most specific (79%) but had modest sensitivity (31%). Detection of EGFR‐positive cells alone and in combination with UPs was inferior to that for UPIa/UPII. Combinations of urothelial markers are superior to single urothelial or epithelial markers in detecting CTCs in bladder cancer patients. Further efforts are under way to confirm the potential predictive value of these markers in a prospectively designed study of a larger cohort of patients. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Heparin-binding epidermal growth factor-
Heparin-binding epidermal growth factor-like growth factor isoforms and epidermal growth factor receptor/ErbB1 expression in bladder cancer and their relation to clinical outcome
✍ Christopher Kramer; Kai Klasmeyer; Hans Bojar; Wolfgang A. Schulz; Rolf Ackerman 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 313 KB

## Abstract ## BACKGROUND. Cleavage of membrane‐anchored heparin‐binding epidermal growth factor‐like growth factor (proHB‐EGF) yields a soluble HB‐EGF isoform (sHB‐EGF), which is an activating epidermal growth factor receptor (EGFR) ligand and a C‐terminal fragment HB‐EGF‐C acting directly in the

Epidermal growth factor receptor gene am
Epidermal growth factor receptor gene amplification and expression in head and neck cancer cell lines
✍ Dr. Ralph R. Weichselbaum; Edward J. Dunphy; Michael A. Beckett; Allen G. Tybor; 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 485 KB

We studied epidermal growth factor receptor (EGFR) gene amplification and expression in 11 early passage human head and neck carcinoma cell lines. Three cell lines demonstrated EGFR gene amplification and 10 lines showed an increase in EGFR mRNA when compared with normal keratinocytes, placenta, and

Differential expression of epidermal gro
Differential expression of epidermal growth factor receptor in human head and neck cancers
✍ Li Dao Ke; Karen Adler-Storthz; Gary L. Clayman; Alfred W. K. Yung; Zhuo Chen 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 165 KB 👁 2 views

## Background: Over-expression of human epidermal growth factor receptor (egfr) is associated with a variety of human malignancies, including head and neck cancer. it has also been studied for its effect on cancer cell responses to chemotherapy. to accurately measure changes in egfr expression that

Expression of estrogen, progesterone and
Expression of estrogen, progesterone and epidermal growth factor receptors in primary and metastatic breast cancer
✍ Ton van Agthoven; Mieke Timmermans; Lambert C. J. Dorssers; Sonja C. Henzen-Logm 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 French ⚖ 473 KB

The prognostic value of epidermal growth factor receptor (EGFR) expression and its biological role in estrogen receptorpositive (ER+) and ER-negative (ER-) primary breast cancer is controversial. In this study, distributions of ER, progesterone receptor and EGFR have been established using immunohis