Colorimetric Detection of DNA Polymerase
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J. Venegas; A. Solari
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Article
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1994
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Elsevier Science
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English
โ 442 KB
A nonradioactive method is developed to detect DNA polymerase activity after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis containing gapped DNA as template. The technique is based on the use of digoxigenin- or biotin-labeled deoxynucleotides during DNA synthesis, and their detecti