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Native polyacrylamide gel electrophoresis of membrane proteins: Glutaminase detection after in situ specific activity staining

✍ Scribed by Juan C. Aledo; Simón Gómez-Biedma; Juan A. Segura; Manuel Molina; Ignacio Núñez de Castro; Javier Márquez


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
681 KB
Volume
14
Category
Article
ISSN
0173-0835

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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m