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Detection of canine cytokine gene expression by reverse transcription-polymerase chain reaction

✍ Scribed by E. Pinelli; S.Y. van der Kaaij; R. Slappendel; C. Fragio; E.J. Ruitenberg; W. Bernadina; V.P.M.G. Rutten


Book ID
117336919
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
82 KB
Volume
69
Category
Article
ISSN
0165-2427

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A powerful method to amplify reverse-transcribed RNA, the polymerase chain reaction can be used to measure cytokine gene transcription in a small number of cells, or in cases where there is low mRNA copy number. This technique may be used to obtain qualitative or quantitative determinations of cytok