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Detection of BK virus in urine by polymerase chain reaction: a comparison of DNA extraction methods

✍ Scribed by A Behzadbehbahani; P.E Klapper; P.J Vallely; G.M Cleator

Book ID
114140432
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
257 KB
Volume
67
Category
Article
ISSN
0166-0934

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A freeze-thaw method was used to release DNA from acetone-killed, dried Brucella abortus S19 cells for polymerase chain reaction. A 635 bp fragment of a 43 kd membrane protein gene was amplified and detected by agarose gel electrophoresis. The sensitivity of the detection was as little as 100 fg of