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Rapid, sensitive detection ofBrucella abortusby polymerase chain reaction without extraction of DNA

✍ Scribed by A. Fekete; J. A. Bantle; S. M. Halling; M. R. Sanborn

Book ID
104647188
Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
564 KB
Volume
4
Category
Article
ISSN
0951-208X

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✦ Synopsis

A freeze-thaw method was used to release DNA from acetone-killed, dried Brucella abortus S19 cells for polymerase chain reaction. A 635 bp fragment of a 43 kd membrane protein gene was amplified and detected by agarose gel electrophoresis. The sensitivity of the detection was as little as 100 fg of cells/sample.

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