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Detection of avocado sunblotch viroid in chloroplasts of avocado leaves by in situ hybridization

✍ Scribed by M. I. Lima; M. E. N. Fonseca; R. Flores; E. W. Kitajima

Book ID
105170638
Publisher
Springer Vienna
Year
1994
Tongue
English
Weight
502 KB
Volume
138
Category
Article
ISSN
1432-8798

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Detection of avocado sunblotch viroid (A
Detection of avocado sunblotch viroid (ASBV) by dot-spot self-hybridization with a [32P]-labelled ASBV-RNA
✍ Arie Rosner; Sara Spiegel; Miriam Alper; Moshe Bar-Joseph πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 549 KB

RNA was extracted from plants infected with avocado sunblotch viroid (ASBV) and was analyzed by electrophoresis in polyacrylamide gel. The ASBV related fraction was eluted from the gel, labelled with [(32)P] using polynucleotide kinase and used as a probe for hybridization with a purified ASBV-RNA p