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Detection of avocado sunblotch viroid by hybridization with synthetic oligonucleotide probes

โœ Scribed by M. Bar-Joseph; D. Segev; S. Twizer; A. Rosner


Book ID
119114281
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
494 KB
Volume
10
Category
Article
ISSN
0166-0934

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RNA was extracted from plants infected with avocado sunblotch viroid (ASBV) and was analyzed by electrophoresis in polyacrylamide gel. The ASBV related fraction was eluted from the gel, labelled with [(32)P] using polynucleotide kinase and used as a probe for hybridization with a purified ASBV-RNA p

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## Abstract The abilities of hybridization probes to detect all human adenovirus types and to identify enteric adenovirus types were evaluated. The efficiency of hybridization was compared to other tests currently in routine laboratory use on clinical specimens from young children with gastroenteri