Detection of antibodies to total histones and their subfractions in systemic lupus erythematosus patients and their asymptomatic relatives
β Scribed by Y. Shoenfeld; G. Segol; O. Segol; B. Neary; A. Klajman; B. D. Stollar; D. A. Isenberg
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 638 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0004-3591
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Two common cross-reacting anti-DNA antibody idiotypes designated 1616 and 32/15, previously identified in the serum of patients who have systemic lupus erythematosus, were found in 24% and 7%, respectively, of 147 first-degree relatives. These findings imply that high-frequency germ-line genes exist
A 96-well microplate ELISA for the detec-native E. coli DNA, calf thymus DNA, and tion of antibodies to DNA is described. A heat-denatured DNA. Using native E. coli number of buffers and precoating treat-DNA, virtually none of 35 normal sera had ments were used to evaluate the optimal any detectable