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Detection of a Single-Locus Gene on Channel Catfish Chromosomes by In-Situ Polymerase Chain Reaction

✍ Scribed by Quiyang Zhang; Richard K Cooper; Terrence R Tiersch


Book ID
117485512
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
434 KB
Volume
118
Category
Article
ISSN
1096-4959

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Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specificsequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose