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In Situ Polymerase Chain Reaction Technique Revealed by Flow Cytometry as a Tool for Gene Detection

✍ Scribed by D. Gibellini; G. Zauli; M.C. Re; G. Furlini; S. Lolli; A. Bassini; C. Celeghini; M. Laplaca


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
569 KB
Volume
228
Category
Article
ISSN
0003-2697

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A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t