Amplification and detection of a Y-chrom
β
Earl A. Timm Jr.; Ed Podniesinski; Carleton C. Stewart; Larry Duckett; John Card
π
Article
π
1995
π
John Wiley and Sons
π
English
β 554 KB
A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t