𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of a single base substitution in a single cell using the LightCycler™

✍ Scribed by Gerard Pals; Chen Young; Hui Sheng Mao; Maria J. Worsham

Book ID
117331064
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
815 KB
Volume
47
Category
Article
ISSN
0165-022X

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Detection of Single-Base Changes Using a
Detection of Single-Base Changes Using a Bioluminometric Primer Extension Assay
✍ Pål Nyrén; Samer Karamohamed; Mostafa Ronaghi 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 134 KB

A rapid bioluminometric technique for real-time detection of known single-base changes is presented. The concept relies on the measurement of the difference in primer extension efficiency by a DNA polymerase of a matched over a mismatched 3' terminal. The rate of the DNA polymerase-catalyzed primer