Detection of a novel HLA-DQ specificity

✍ Scribed by Hiroshi Kojima; Yu'ichiro Fukasawa; Naoshi Ishikawa; Yasutaka Tajima; Akemi Wakisaka; Miki Aizawa

• Book ID: 104735083
• Publisher: Springer-Verlag
• Year: 1988
• Tongue: English
• Weight: 253 KB
• Volume: 27
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00351090

✦ Synopsis

At the Ninth International Histocompatibility Workshop (Munich, 1984), DP, DQ, and DR antigens were officially recognized as the class II gene products of the HLA complex (Bodmer et al. 1984). The DP, DQ, and DR loci were considered to have at least 6, 3, and 14 (2 more in a proximal locus) alleles, respectively. DQ molecules have been revealed to be the human homolog of mouse A molecules (Bono and Strominger 1982, Giles et al. 1983), although only three alleles have been identified despite the fact that the mouse A system is highly polymorphic (Klein et al. 1983). Recent findings of twodimensional gel analyses, however, have demonstrated that the DQ system is as polymorphic as the mouse A system (de Kretser et al. 1982, Shackelford et al. 1983, Maeda et al. 1984, Ishikawa et al. 1985). Nevertheless, no serological reagent was available to detect the fine specificity corresponding to these observations, except for a few mouse monoclonal antibodies (mAbs) such as TA10 and HU-23, both of which dissected DQw3 antigens associated with Dw4 and Dw5 from those associated with other D specificities (Kasahara et al. 1983, Maeda 1984). Thus, only three kinds of alloantisera detecting the supertypic determinants on DQ molecules were utilized to define the phenotypes of the DQ antigens as DQwl, w2, and w3. Moreover, the limitation of antisera made it difficult to define the DQ antigens associated with the Dwl5-DR4 and certain Dw8-DRw8 specificities, which do not belong to any of the DQw 1, w2, and w3 families (Schreuder and Degos 1984).

To investigate the DQ system, we produced mouse mAb HU-46, which defines a novel DQ specificity,