Detection of a novel HLA-DQ specificity: serological and immunochemical analyses by a monoclonal antibody

✍ Scribed by Naoshi Ishikawa; Hiroshi Kojima; Tsuguyo Nakayama; Hiroshi Kunikane; Shuichi Hawkin; Yu-ichiro Fukasawa; Hitoshi Ikeda; Kazumasa Ogasawara; Masanori Kasahara; Yasutaka Tajima; Yukio Kakuta; Akemi Wakisaka; Miki Aizawa

• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 725 KB
• Volume: 26
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00365903

✦ Synopsis

A monoclonal antibody (mAb) with a novel human B-cell allospecificity was produced by immunizing a C3H/He mouse with the human B lymphoblastoid cell line EBV-Wa (HLA-DR4/Dw15/DQblank homozygous). The mAb, termed HU-46, reacted with B cells from not only DR4/Dwl5-positive individuals but also certain DRw8/Dw8-positive ones whose DQ phenotypes had not yet been defined. Two-dimensional gel analyses indicated that the mAbrecognized class II antigens which were encoded by the HLA-DQ locus. Furthermore, in genetic analysis, the gene encoding the class II antigen detected by HU-46 met the Hardy-Weinberg condition as a fourth allele of the DQ locus. We provisionally labeled this novel DQ specificity DQWa.