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Detection byin-situfluorescence of short, single-copy sequences of chromosomal DNA

โœ Scribed by Tong Zhu; Liang Shi; Paul Keim


Book ID
112822224
Publisher
Springer
Year
1995
Tongue
English
Weight
555 KB
Volume
13
Category
Article
ISSN
0735-9640

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Recombinant lambda bacteriophage clone H3 containing a human DNA segment of 14.9 kb present in one or two copies per haploid genome was isolated. In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling (53% of cells) of band p36 of ch