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Detection and sequence analysis of tryptic peptides by microcolumn liquid chromatography-tandem mass spectrometry using an electrospray interface

✍ Scribed by Mark Hail; Sydell Lewis; Ian Jardine; Jinping Liu; Milos Novotny

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
501 KB
Volume
2
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

Fused‐silica packed LC capillaries were interfaced with a triple quadrupole mass spectrometer through the electrospray ionization device. The coupling of these powerful techniques is suitable for structural characterization of constituents of peptide maps generated at high sensitivity. Sequence determinations appear possible at the femtomole level following this approach.

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