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Detection and Quantification ofCitrobacter freundiiandC. braakiiby 5′-Nuclease Polymerase Chain Reaction

✍ Scribed by Eva Kaclíková; Klára Krascsenicsová; Domenico Pangallo; Tomáš Kuchta


Book ID
105996311
Publisher
Springer
Year
2005
Tongue
English
Weight
136 KB
Volume
51
Category
Article
ISSN
0343-8651

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A non-gel-based quantification assay based on competitive PCR and bioluminometric detection has been developed. Samples containing human immunodeficiency virus type 1 (HIV-1) DNA and three quantitative standards at discrete concentrations were coamplified by PCR with primers annealing in the polymer