Detection and quantification of hepatitis B virus DNA by SYBR green real-time polymerase chain reaction
β Scribed by D. Olioso; M. Boaretti; M. Ligozzi; G. Lo Cascio; R. Fontana
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 396 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0934-9723
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The polymerase chain reaction (PCR) technique has been utilized for the detection of hepatitis B virus (HBV) DNA, and several factors related to the selection of primer pairs for the PCR amplification have been demonstrated. The sensitivity of the PCR assay was compared with that of slot-blot hybrid
## Abstract Hepatitis B virus (HBV) DNA was assayed using the polymerase chain reaction in serum samples of 116 hepatitis B surface antigen (HBsAg) carriers, including 30 positive for hepatitis B e antigen (HBeAg) and 86 negative for HBeAg. In the HBeAgβpositive group, all were positive for HBV DNA
## Abstract A sensitive and reproducible realβtime PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an βinβhouseβ qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B pati