## Abstract For the detection of anabolic steroid residues in bovine urine, a highly sensitive liquid chromatographic/electrospray ionization tandem mass spectrometric (LC/ESI‐MS/MS) method was developed using both positive and negative ionization. For four compounds the ESI mode was not sensitive
Detection and identification of anabolic steroids in bovine urine by gas chromatography—mass spectrometry
✍ Scribed by B. Le Bizec; M.-P. Montrade; F. Monteau; F. Andre
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 696 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
Wlthm the scope of the national plan for hormone control m France, a study was made to develop a system for the control of the Illegal use of anabohc steroids for fattemng purposes m ammal productlon A screenmg procedure for these residues m bovme urme was developed, based on Cls sohd-phase extration, s&a gel pur&atlon and gas chromatogmphw-mass spectrometic analyw wth selected IOU momtormg All the method parameters are dwxssed, espewlly the enzymatic and chermcal hydrolysis steps and the choux of denvatwes Keywords Gas chromatography-mass spectrometry, Anabohc sterouis, Bovme urme, Hormones, Urme
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High resolution gas chromatography-mass spectrometry has been used to detect anabolic steroids in urine. With fused silica capillary columns connected directly to mass spectrometer, the anabolic steroids could be detected after extraction, hydrolysis, and derivatization with a sensitivity betterthen
## Abstract A fast and sensitive method for the comprehensive screening of anabolic agents and other banned doping substances using gas chromatography/tandem mass spectrometry (GC/MS/MS) with an external ionization ion trap mass spectrometer is presented. The method takes advantage of the resolving