Detection and identification of 2-ethyl-3-oxohexanoic acid-a new 3-oxocarboxylic acid in human serum and urine

✍ Scribed by Liebich, H. M. ;Huesgen, G. ;Pickert, A. ;Stierle, U. ;Wöll, J.

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 199 KB
• Volume: 6
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240060912

✦ Synopsis

The heptafluorobutyryl derivatives of some steroid standards were analyzed on a 25 m X 0.32 mm OV-1 fused silicacolumn. The effluent was split in the ratio : FID 5 : ECD 1 by using split arms of 20 cm X 0.32 mm ID and 20 X 0.25 mm ID respectively. Make-up gas was nitrogen with a flow-rate of 22 ml min-'. The amount injected was 1 ng per compound corresponding to approximately 160 pg for ECD detection. Interesting in this analysis is the increasing ECD response with increasing fluorine content of the derivatives.

The response of El (peak 2), E2 (peak l), E3 (peak3), and E4 (peak 8) containing respectively one, two, three, and four heptafluorobutyryl groups is therefore approximately 1 :2:3:4.Thisallows easy identification of these compounds in complex biological fluids. The response of these compounds in the FIB trace is equal.

4 Conclusion

Multiple detector operations can easily be performed with polyimide split devices. The devices described are suitable for numerous applications.