✦ LIBER ✦

Detection and determination of factor C — a regulatory protein —inStreptomycesstrains by antiserum and monoclonal antibody

✍ Scribed by F. Szeszák; S. Vitális; F. Tóth; G. Valu; J. Fachet; G. Szabó

Publisher: Springer
Year: 1990
Tongue: English
Weight: 266 KB
Volume: 154
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00249182

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✦ Synopsis

Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34,000 or 70,000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy+ strains were usually good producers of factor C while Amy- were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.

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