Generalized autoimmunity of the nervous system (GANS) induced by a recombinant protein composed of major pathogenic determinants of MBP, IRBP, and P2 protein: Suppression of inflammation by a monoclonal antibody against activated rat T line cells
✍ Scribed by H. Schluesener
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 426 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
We have developed a new model of generalized autoimmunity of the rat nervous system to study differential immunoregulation, barrier-function, and parenchymal inflammatory processes. We designed a mul ticomponen t synthetic gene encoding major pathogenic determinants for Lewis rats of myelin basic protein (MBP), interphotoreceptor retinoid binding protein (IRBP), abd Pz protein. Immunization with the recombinant protein induces a monophasic disease with inflammatory lesions in the eye, brain, spinal cord, and peripheral nerves. Rats recovered from GANS were tolerant against the induction of experimental autoimmune encephalomyelitis (EAE) , neuritis (EAN), and uveoretinitis (EAU) by immunization with synthetic autoantigen-peptides/CFA.
To demonstrate an application of GANS we have used a monoclonal antibody raised against encephalitogenic rat T lymphocytes. We show that this monoclonal antibody is suppressing not only inflammatory cell infiltration of brain and spinal cord, but as well of the eyes and the peripheral nervous system.