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Detection and cloning of epidermal zinc-α2-glycoprotein cDNA and expression in normal human skin and in tumors

✍ Scribed by Gang Lei; Istvan Arany; Peter Selvanayagam; Srinivasan Rajaraman; Sandhya Ram; Henry Brysk; Stephen K. Tyring; Miriam M. Brysk

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
172 KB
Volume
67
Category
Article
ISSN
0730-2312

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✦ Synopsis

Zinc-alpha 2-glycoprotein (Zn alpha 2gp) is almost ubiquitous in body fluids, and its antibody labels the corresponding secretory epithelia. We have found that Zn alpha 2gp is also expressed in human epidermis. We cloned the Zn alpha 2gp cDNA by screening our cDNA library, derived from epidermal keratinocytes, with a probe for prostate Zn alpha 2gp. It had complete nucleic acid sequence homology with that from prostate, including the signal peptide. Just as Zn alpha 2gp expression is higher in more differentiated breast tumors, so in skin tumors the highest mRNA levels occurred in the normal controls, the lowest in basal cell carcinomas (the least differentiated epidermal tumor type), and intermediate levels in squamous cell carcinomas and Merkel cell carcinomas. A similar increase in Zn alpha 2gp gene expression with differentiation was observed when epidermal keratinocytes were cultured in media that varied in cellular maturation potential.

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