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Detection and characterization of native β-endorphin1–31 in bovine pituitary using electrospray ionization, liquid secondary ion and tandem mass spectrometry

✍ Scribed by Lin Yan; Jih-Lie Tseng; Dominic M. Desiderio

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
637 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

The molecular ion of intact B-endorphinbovlne, (BE), which was extracted from bovine pituitary, was determined by electrospray ionization mass spectrometry. Liquid secondary ion mass spectrometry determined the molecular ma-of three peptides produced by trypsin digestion of BE, and tandem mass spectrometry was used to determine the amino acid sequence of the tryptic peptide BE20-24. These data, in combination, were used to characterize BE in bovine pituitary.

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