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Detecting Enzymatic Activity in Cells Using Fluorogenic Substrates

โœ Scribed by Haugland, Richard P.

Book ID
115465649
Publisher
Informa plc
Year
1995
Tongue
English
Weight
727 KB
Volume
70
Category
Article
ISSN
1052-0295

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A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubatio