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Detecting DNA-binding of proteins in vivo by UV-crosslinking and immunoprecipitation

✍ Scribed by Lemin Zhang; Keqin Zhang; Ralf Prändl; Fritz Schöffl

Book ID
116289048
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
601 KB
Volume
322
Category
Article
ISSN
0006-291X

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Crosslinking of DNA to nuclear lamina proteins by UV irradiation in vivo
✍ Z. Galcheva-Gargova; G. N. Dessev 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 319 KB

We have been able to demonstrate that a fraction of DNA becomes crosslinked to nuclear lamina shells isolated from Ehrlich ascites tumour cells irradiated with UV light. Terminal labeling of short DNA fragments covalently attached to proteins reveals that DNA has become crosslinked to all three lami