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Crosslinking of DNA to nuclear lamina proteins by UV irradiation in vivo

✍ Scribed by Z. Galcheva-Gargova; G. N. Dessev

Book ID: 102302620
Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 319 KB
Volume: 34
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.240340303

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✦ Synopsis

We have been able to demonstrate that a fraction of DNA becomes crosslinked to nuclear lamina shells isolated from Ehrlich ascites tumour cells irradiated with UV light. Terminal labeling of short DNA fragments covalently attached to proteins reveals that DNA has become crosslinked to all three lamins and to a protein comigrating with vimentin.

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