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Detailed marker chromosome analysis in cell line U-BLC1, established from transitional-cell carcinoma of the bladder

✍ Scribed by Jochen Bruch; Gudrun Wöhr; Silke Brüderlein; Gotthold Barbi; Hubertus Wolter; Christa Dixkens; Torsten Mattfeldt; Peter Möller; Thomas Paiss; Richard Hautmann; Walther Vogel; Horst Hameister

Publisher
John Wiley and Sons
Year
1999
Tongue
French
Weight
301 KB
Volume
80
Category
Article
ISSN
0020-7136

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✦ Synopsis

A permanent cell line, U-BLC1, was established from a primary transitional-cell carcinoma, TCC, of the urinary bladder. Karyotype analysis showed the line to be highly aberrant, with a near-triploid chromosome number of 68 to 73. Comparative genomic hybridization revealed some distinct differences between the primary tumor and the established cell line. Karyotype analysis showed 3 marker chromosomes with homogeneously staining regions, HSRs, in the cell line. The HSRs were isolated by microdissection and the microdissection probes were hybridized to normal metaphase chromosomes. The HSRs contain sequences known to be frequently involved in amplification in transitional-cell carcinoma of the bladder, 6p22, 7p11-p12, 9p23-pter, and one region not yet reported to be amplified in primary TCC of the bladder, 1p31-p32. A candidate-gene approach showed that in the region 7p11-p12 the EGFR locus is amplified and highly expressed. Int.

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