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Detailed deletion mapping on chromosome arm 12q in human pancreatic adenocarcinoma: Identification of a 1-cM region of common allelic loss

✍ Scribed by Mitsuhiro Kimura; Tadayoshi Abe; Makoto Sunamura; Seiki Matsuno; Akira Horii

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 530 KB
Volume: 17
Category: Article
ISSN: 1045-2257
DOI: 10.1002/(sici)1098-2264(199610)17:2<88::aid-gcc3>3.0.co;2-x

No coin nor oath required. For personal study only.

✦ Synopsis

As a first step toward understanding molecular mechanisms in human pancreatic carcinogenesis, we searched for the location of tumor suppressor genes by examining loss of heterozygosity (LOH) in 44 pancreatic cancer specimens. W e used 46 microsatellite markers that spanned all of the autosomes. Frequent LOH was observed in six chromosomal regions: in chromosome arms Ip (32%), 6q (37%), 9p (50%). 12q (30%), 17p (59%), and 18q (35%). Because chromosome arm 12q is a reported target for allelic loss in some other cancers, we focused on this region with 66 primary specimens and identified the minimal common region of allelic loss within a I -cM interval in I2q22-q23. I. Microsatellite instability (MI) was also examined in this study, and the incidence of MI( +) cases, in which MI of two or more microsatellite loci was detected, was 61% (27 of 44 informative cases). In pancreatic tumors with MI( +), mutations of the transforming growth factor p receptor II (RII) gene were not detected.

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