Detailed deletion mapping of chromosome segment 17q12-21 in sporadic breast tumours

✍ Scribed by Maria A. Nagai; Lidia Yamamoto; Sibeli Salaorni; Mércia M. Pacheco; M. Mitzi Brentani; Edson M. Barbosa; Ricardo R. Brentani; Sylvie Mazoyer; Simon A. Smith; Bruce A. J. Ponder; Lois M. Mulligan

• Book ID: 102846781
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 569 KB
• Volume: 11
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870110109

✦ Synopsis

Linkage studies have indicated that a gene on chromosome arm 17q. designated BRCA I , confers susceptibility t o familial breast and ovarian cancer. To investigate the possible involvement of the BRCA I gene in sporadic breast cancer we have analysed loss of heterozygosity (LOH) in a panel of 100 sporadic primary breast tumours using I0 PCR-based polymorphic markers from 17q 12-2 I. Allele losses were detected in 40 of I00 tumours informative for at least one of the markers analysed. Of these 40 deleted tumours, 27 showed partial or interstitial loss on I7q. The pattern of LOH in the tumours with partial or interstitial LOH revealed three putative distinct deleted regions on 17q I 2-2 I . The first lies on the proximal long arm between D I75250 and THRA I ; the second one lies between D I75776 and D 175579, the region containing the BRCA I gene; and the third is telomeric to D175733. The most frequently deleted region overlaps with the minimal region containing the BRCAI gene, suggesting that this gene might also be associated with the development or progression of a proportion of sporadic breast tumours.