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Design, synthesis and structural characterization of model heterodimeric coiled-coil proteins

✍ Scribed by ZHU, BING-YAN ;ZHOU, NIAN E. ;SEMCHUK, PAUL D. ;KAY, CYRIL M. ;HODGES, ROBERT S.


Book ID
115099311
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
784 KB
Volume
40
Category
Article
ISSN
0367-8377

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✍ Masaru Hoshino; Yuji Goto; Noboru Yumoto; Susumu Yoshikawa πŸ“‚ Article πŸ“… 1997 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 852 KB

As a model for analyzing the role of charge repulsion in proteins and its shielding by the solvent, we designed a peptide of 27 amino acid residues that formed a homodimeric coiled-coil. The interface between the coils consisted of hydrophobic Leu and Val residues, and 10 Lys residues per monomer we