✦ LIBER ✦

Design of coupled reactions for simplification of bioluminescence analysis

✍ Scribed by Brolin, S. E. ;Naeser, P. ;Wettermark, G.

Publisher: John Wiley and Sons
Year: 1989
Weight: 366 KB
Volume: 4
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170040159

No coin nor oath required. For personal study only.

✦ Synopsis

Luminescence analysis may be applied t o many substances by arranging a prior reaction producing a species entering the light-emitting reaction. Under favourable conditions the two consecutive reactions are carried out simultaneously as a one-step procedure. In a bioluminescence assay, luciferase stability is frequently a problem, making it desirable t o develop analytical schemes where the analytical response becomes largely independent of any impaired luciferase activity. The value of maximal emission or an approached steady-state level is a convenient and usually well-defined analytical parameter. When recording this level it is important t o design the participating reactions in a way that compensates for changes in luciferase activity.

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