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Design of Affinity Tags for One-Step Protein Purification from Immobilized Zinc Columns

โœ Scribed by Richard S. Pasquinelli; Rex E. Shepherd; Richard R. Koepsel; Adam Zhao; Mohammad M. Ataai


Book ID
109388327
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
237 KB
Volume
16
Category
Article
ISSN
8756-7938

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This work demonstrates that proper selection of a metal ion and chelating ligand enables recovery of a his(6)-tagged protein from canola (Brassica napus) extracts by immobilized metal affinity chromatography (IMAC). When using Co(2+) with iminodiacetate (IDA) as the chelating ligand, beta-glucuronid