The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins
β Scribed by Schmidt, Thomas GM; Skerra, Arne
- Book ID
- 109946202
- Publisher
- Nature Publishing Group
- Year
- 2007
- Tongue
- English
- Weight
- 293 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1750-2799
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract A multiple vector system for the intracellular highβlevel production of affinity tagged recombinant proteins in __Bacillus megaterium__ was developed. The Nβ and Cβterminal fusion of a protein of interest to a Strep II and a His~6~βtag is possible. Corresponding genes are expressed unde
## Abstract Although the concept of affinity purification using synthetic ligands had been utilized for many years, there are few articles related to this research area, and they focus only on the affinity purification of specific protein by a defined library of synthetic ligands. This study presen