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Description and properties of bacteriophage Lambda vectors useful for the cloning of EcoRI DNA fragments

✍ Scribed by Kourilsky, P.; Perricaudet, M.; Gros, D.; Garapin, A.; Gottesman, M.; Fritsch, A.; Tiollais, P.

Book ID
122738091
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
844 KB
Volume
60
Category
Article
ISSN
0300-9084

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In vitro recombination techniques were used to construct hybrid plasmids between pBR 322 and different DNA fragments derived from pML 21 and the E. coli chromosome. Some of the resulting hybrid plasmids express the tetracycline resistance gene of pBR 322, depending on the DNA fragment which has been