Use of differential agar media for detection of cloned DNA fragments in the tetracycline and chloramphenicol resistance genes of pBR322

The expression of tetracycline resistanc

The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR 322

✍ Widera, Georg ;Gautier, Fritz ;Lindenmaier, Werner ;Collins, John 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 429 KB

In vitro recombination techniques were used to construct hybrid plasmids between pBR 322 and different DNA fragments derived from pML 21 and the E. coli chromosome. Some of the resulting hybrid plasmids express the tetracycline resistance gene of pBR 322, depending on the DNA fragment which has been