✦ LIBER ✦

Derivatization reactions for gas chromatography/mass spectrometry determination of N3-methyl-5′-deoxy-5-fluorouridine

✍ Scribed by Carlo G. Zambonin; Silvio Mastrolitti; Francesco Palmisano

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 226 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199709)11:14<1529::aid-rcm20>3.0.co;2-h

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✦ Synopsis

N,O-bis(trimethylsilyl)trifluoroacetamide, N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide and trifluoroacetic anhydride have been investigated as derivatizing agents for GC/MS determination of N 3 -methyl-5'-deoxy-5-fluorouridine (N 3 -Me-5'-dFUR), a metabolic product of the anticancer pro-drug doxifluridine. Mass spectral information is given and some fragmentation patterns are discussed. Relative merits of the three derivatizing agents are also discussed. Examples of applications to spiked serum and urine samples are reported. Detection limits of the order of 10 nM were obtained when operating in selected ion recording mode.

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