Dependence of Staphylococcus epidermidis on non-transferrin-bound iron for growth
✍ Scribed by Sanna Matinaho; Leni von Bonsdorff; Ari Rouhiainen; Maarit Lönnroth; Jaakko Parkkinen
- Book ID
- 117516775
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 263 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0378-1097
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direct method for quantification of non-transferrin-bound iron has been developed. This assay relies on the use of a large excess of a low affinity ligand (nitrilotriacetic acid, NTA) which removes and complexes all low molecular weight iron and iron nonspecifically bound to serum proteins. Iron bou
We introduce a method for monitoring non-transferrin-bound iron (NTBI), a labile and potentially toxic form of serum iron associated with imbalanced iron metabolism. The assay employs fluorescein-labeled apotransferrin (Fl-aTf), which undergoes fluorescence quenching upon binding iron. It has the ad